Estimation of virus - neutralizing antibody in
نویسنده
چکیده
Virus-neutralizing antibodies are seldom estimated in routine diagnostic laboratories because methods involving replicate series of tubed cell monolayers are so demanding of time and materials. Simplified methods for performing neutralization tests have been described, but these were generally developed for specific purposes and have required various forms of specialized apparatus. One of the earliest techniques was introduced by Melnick and Opton (1956) for the assay of polio antibodies in monkey kidney cells. Plastic panels were used and results were determined by the observation of metabolic inhibition. When microtitration apparatus became commercially available, neutralization tests were performed in plastic microplates. Sever (1961) described a system for measuring polio antibodies, whilst Schmidt, Lennette, and Hanahoe (1966) used a micro-test for the detection of reovirus antibodies in the sera of laboratory animals. Both of these methods also depended upon the metabolic inhibitory action of virus upon the cell cultures. Moreau and Furesz (1967) employed a micro-technique for the titration of measles antibodies. The extent of cytopathic effect produced by the virus was observed microscopically. Most of the methods involved the rather inconvenient practice of sealing the microplate wells with mineral oil, followed by incubation in humidified cabinets, gassed with a mixture ofcarbon dioxide and air. These precautions were required to enable bicarbonate buffers to maintain the culture media at a suitable pH for cell growth. Another disadvantage of micro-testing has been the necessary chemical treatment of microplates to remove cytotoxic factors. The aim of this paper is to present a simplified micro-technique for the estimation of virus-neutra-
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تاریخ انتشار 2004